LIttoral ENvironnement et Sociétés (LIENSs) - UMR 7266

benthic meiofauna

Thierry Guyot — 2021-01-04T10:22:36Z

BENTHIC MEIOFAUNA :

Meiofauna consist of microscopically small, motile animals (size ranging from 44 to 500 µm) occurring in and on soft substrates of both marine and freshwater ecosystems. They are known for their high abundance within coastal ecosystems. Nematodes generally dominate meiofauna communities in coastal intertidal ecosystems, often followed by benthic copepods as the second most abundant group. Nematodes occur from polar to tropical regions and from sandy beaches to deep-sea sediments. Their abundance is very high in intertidal soft-bottom coastal ecosystems, however this abundance can be quite variable (10⁵ to 10⁸ individuals.m^-2). Benthic copepods typically occur in habitats with medium-to-fine sand, where their abundance can be slightly higher than those of nematodes. Other groups of meiofauna, such as ostracods and polychaetes, are generally less abundant in soft-bottom intertidal habitats.

BENTHIC MEIOFAUNA STUDIED IN THE TEAM BIOFEEL :

Researchers from the team BIOFEEL mostly study two major groups of meiofauna : the nematodes, and the benthic copepods. Nematodes are characterized by four major feeding types, which biomass change depending on abiotic and biotic parameters : selective deposit feeders (which mostly rely on bacteria), non-selective deposit feeders, epigrowh feeders (which mostly rely on benthic microalgae, particularly diatoms) and omnivores/carnivores. Species from these different groups can be identified using the shape of their mouth, as this shape is closely related to their feeding behavior. Benthic copepods are generally dominated by harpacticoid and cyclopoid copepods. If nematodes can migrate into the sediment, copepods very mostly occur in the very first mm of the sediment.

RESEARCH CARRIED OUT IN THE TEAM BIOFEEL ABOUT BENTHIC MEIOFAUNA :

Determination of the food resources used by nematodes and benthic copepods using trophic markers (stable isotopes, fatty acids).
Determination of community structure of nematode assemblages.

RESEARCHERS FROM THE TEAM BIOFEEL WORKING ON BENTHIC MEIOFAUNA :

Christine Dupuy
Denis Fichet
Benoit Lebreton
Luuk van der Heijden

REFERENCE PUBLICATIONS :

Giere O., 2009. Meiobenthology. The microscopic fauna in aquatic sediments. Springer-Verlag, Berlin, 328 p.
Heip C., Vincx M., Vranken G., 1985. The ecology of marine nematodes. Oceanography and Marine Biology. An Annual Review 23, 399-489.
Hicks G.R.F., Coull B.C., 1983. The ecology of marine meiobenthic harpacticoid copepods. Oceanography and Marine Biology. An Annual Review 21, 67-175.
Moens T., Bergtold M., Traunspurger W., 2006. Feeding ecology of free-living benthic nematodes. In : Freshwater nematodes : ecology and taxonomy. Abebe E., Andrassy I., Traunspurger W. (Eds.), Wallingford & Cambridge, USA, CABI Publishing : 105-131.

MAJOR PUBLICATIONS OF THE TEAM BIOFEEL :

Dupuy C., Nguyen Thanh H., Mizrahi D., Jourde J., Bréret M., Agogué H., Beaugeard L., Bocher P., 2015. Structure and functional characteristics of the meiofauna community in highly unstable intertidal mudbanks in Suriname and French Guiana (North Atlantic coast of South America). Continental Shelf Research 110, 39-47.
Lebreton B., Richard P., Galois R., Radenac G., Brahmia A., Colli G., Grouazel M., André C., Guillou G., Blanchard G.F., 2012. Food sources used by sediment meiofauna in an intertidal Zostera noltii seagrass bed : a seasonal stable isotope study. Marine Biology 159, 1537-1550.
Pascal P.-Y., Dupuy C., Richard P., Haubois A.-G., Niquil N., 2008. Influence of environment factors on bacterial ingestion rate of the deposit-feeder Hydrobia ulvae and comparison with meiofauna. Journal of Sea Research 60, 151-156.
Pascal P.-Y., Dupuy C., Richard P., Mallet C., Armynot du Chatelet E., Niquil N., 2009. Seasonal variation in consumption of benthic bacteria by meio- and macrofauna in an intertidal mudflat. Limnology and Oceanography 54, 1048-1059.
Rzeznik-Orignac J., Boucher G., Fichet D., Richard P., 2008. Stable isotope analysis of food source and trophic position of intertidal nematodes and copepods. Marine Ecology Progress Series 359, 145-150.
Rzeznik-Orignac J., Fichet D., Boucher G., 2003. Spatio-temporal structure of the nematode assemblages of the Brouage mudflat (Marennes Oléron, France). Estuarine, Coastal and Shelf Science 58, 77-88.
Rzeznik-Orignac J., Fichet D., Boucher G., 2004. Extracting massive numbers of nematodes from muddy marine deposits : efficiency and selectivity. Nematology 6, 605-616.
Van der Heijden L.H., Rzeznik-Orignac J., Asmus R.M., Fichet D., Bréret M., Kadel P., Beaugeard L., Asmus H., Lebreton B., 2018. How do food sources drive meiofauna community structure in soft-bottom coastal food webs ? Marine Biology 165, 166.

benthic microalgae

Thierry Guyot — 2021-01-04T10:15:21Z

BENTHIC MICROALGAE :

Benthic microalgae are photosynthetic microorganisms growing in the sediment, contrarily to phytoplankton that develop in the water column. The microphytobenthos can consist of diatoms, cyanobacteria, Chlorophyceae and/or flagellates. Among the diatoms, which largely dominate in intertidal mudflats of the Atlantic coasts, two types can be observed : the epipelic diatoms, which are motile, and the epipsammic diatoms, which are generally smaller and stick to sediment grains with mucilaginous secretions. Epipsammic diatoms generally dominate in the sandy and mixed sediments, while epipelic diatoms dominate in pure mud. These epipelic diatoms migrate to the sediment surface during diurnal low tides to realize the photosynthesis. Thirty-seven species of diatoms have been identified on the Brouage mudflat, which is one of the largest mudflats in the Marennes-Oléron bay.

BENTHIC MICROALGAE STUDIED IN THE TEAM BIOFEEL :

Most of the studies on benthic microalgae are carried out on intertidal bare mudflats, but some studies are also done on other soft-bottom coastal habitats, like seagrass beds, sandflats, and shallow subtidal embayments. Researchers from the team BIOFEEL focus their research on the primary production of benthic microalgae, especially on bare mudflats, by building models of primary production based on remote sensing. Another part of the studies aims at better assessing the role of the microphytobenthos as a food source for the meiofauna, the macrofauna, and for fish (i.e. mullets).

RESEARCH CARRIED OUT IN THE TEAM BIOFEEL ABOUT BENTHIC MICROALGAE :

Quantification of primary production based on modelling.
Determination of the role of benthic microalgae as a food source in the functioning of intertidal and subtidal habitats using trophic markers (stable isotopes, fatty acids).

RESEARCHERS FROM THE TEAM BIOFEEL WORKING ON BENTHIC MICROALGAE :

Christine Dupuy
Vincent Le Fouest
Benoit Lebreton
Raphaël Savelli

REFERENCE PUBLICATIONS :

Blanchard G.F., 2006. Analyse conceptuelle du système de production primaire microphytobenthique des vasières intertidales. Océanis 32, 215-235.
Kromkamp J.C., de Brouwer J.F.C., Blanchard G.F., Forster R., Créach V., 2006. Functioning of microphytobenthos in estuaries. Royal Academy of Arts and Sciences, Amsterdam, 262 p.
MacIntyre H.L., Geider R.J., Miller D.C., 1996. Microphytobenthos : The ecological role of the "secret garden" of unvegetated, shallow-water marine habitats. I. Distribution, abundance and primary production. Estuaries 19, 186-201.
Miller D.C., Geider R.J., MacIntyre H.L., 1996. Microphytobenthos : The ecological role of the Secret Garden of unvegetated, shallow-water marine habitats. II. Role in sediment stability and shallow-water food webs. Estuaries 19, 202-212.
Round F.E., Crawford R.M., Mann D.G., 1990. The Diatoms : biology and morphology of the genera. Cambridge University Press, Cambridge, 747 p.

MAJOR PUBLICATIONS OF THE TEAM BIOFEEL :

Blanchard G.F., Guarini J.-M., Orvain F., Sauriau P.-G., 2001. Dynamic behaviour of benthic microalgal biomass in intertidal mudflats. Journal of Experimental Marine Biology and Ecology 264, 85-100.
Guarini J.-M., Blanchard G.F., Gros P., Gouleau D., Bacher C., 2000. Dynamic model of the short-term variability of microphytobenthic biomass on temperate intertidal mudflats. Marine Ecology Progress Series 195, 291-303.
Haubois A.-G., Sylvestre F., Guarini J.-M., Richard P., Blanchard G.F., 2005. Spatio-temporal structure of the epipelic diatom assemblage from an intertidal mudflat in Marennes-Oléron Bay, France. Estuarine, Coastal and Shelf Science 64, 385-394.
Herlory O., Guarini J.-M., Richard P., Blanchard G.F., 2004. Microstructure of microphytobenthic biofilm and its spatio-temporal dynamics in an intertidal mudflat (Aiguillon Bay, France). Marine Ecology Progress Series 282, 33-44.
Lebreton B., Richard P., Galois R., Radenac G., Pfléger C., Guillou G., Mornet F., Blanchard G.F., 2011. Trophic importance of diatoms in an intertidal Zostera noltii seagrass bed : Evidence from stable isotope and fatty acid analyses. Estuarine, Coastal and Shelf Science 92, 140-153.
Méléder V., Savelli R., Barnett A., Polsenaere P., Gernez P., Cugier P., Lerouxel A., Le Bris A., Dupuy C., Le Fouest V., Lavaud J., 2020. Mapping the intertidal microphytobenthos gross primary production, part I : Coupling multispectral remote sensing and physical modeling. Frontiers in Marine Science 7, 520.
Riera P., Richard P., 1996. Isotopic determination of food sources of Crassostrea gigas along a trophic gradient in the estuarine bay of Marennes-Oléron. Estuarine, Coastal and Shelf Science 42, 347-360.
Savelli R., Méléder V., Cugier P., Polsenaere P., Dupuy C., Lavaud J., Barnett A., Le Fouest V., 2020. Mapping the intertidal microphytobenthos gross Primary production, Part II : Merging remote sensing and physical-biological coupled modeling. Frontiers in Marine Science 7, 521
van der Heijden L.H., Niquil N., Haraldsson M., Asmus R.M., Pacella S.R., Graeve M., Rzeznik-Orignac J., Asmus H., Saint-Béat B., Lebreton B., 2020. Quantitative food web modeling unravels the importance of the microphytobenthos-meiofauna pathway for a high trophic transfer by meiofauna in soft-bottom intertidal food webs. Ecological Modelling 430, 109129.

Mudflats

Thierry Guyot — 2021-01-04T10:00:32Z

MUDFLATS : general description

Mudflats are intertidal or subtidal habitats covered by mud. Mud is a sediment made of clay which particle size is smaller than 63 µm. These particles generally form in estuaries, where fresh- and salt-water mix, which leads fine particles to flocculate into larger particles. These particles are flushed towards the sea and then settle down in areas with low hydrodynamics. Intertidal bare mudflats, located in the shore, are characterized by a very high biological productivity, especially related to the production of benthic microalgae, which grow at the surface of the sediment. This production fuels a complex food web made of microfauna, meiofauna and macrofauna at its first trophic levels. These consumers are food resources for predators like fish and birds. The very high production of benthic microalgae on intertidal bare mudflats benefits to shellfish farming activities, especially oyster and mussel farming, as these two species largely rely on benthic microalgae when they are cultured on or close to intertidal bare mudflats.

MUDFLATS specifically studied by the researchers from the team BIOFEEL :

The researchers from the team BIOFEEL carry out their research on the mudflats located on the Charente-Maritime coastline, located in the Marennes-Oléron bay (Brouage mudflat, Yves cove) and in the Aiguillon bay. Other mudflats are also studied, especially those located along the French Atlantic coast (Bay of Bourgneuf), in the Mont Saint-Michel Bay, in the Seine river estuary, and in the Dutch and the German (Sylt-Rømø Bight) parts of the Wadden Sea. Other studies are carried out in French Guyana and on subtidal mudflats of the shallow water bays located along the Gulf of Mexico in the United-States.

RESEARCH CARRIED OUR ON MUDFLATS :

Research carried out on mudflats aims at understanding their functioning, by studying the structure of the flora and fauna communities which develop in intertidal bare mudflats, or colonize them, and by determining the biotic and abiotic factors structuring these communities. Flows of organic matter between these different communities are quantified and modelized to determine what are the properties of these habitats, and how they change, especially productivity. This research is carried out thanks to several methods, from molecular biology to taxonomy for the study of community structure, and from the study of gut contents to the use of trophic markers (stable isotopes, fatty acids) and models for the quantification of flows of organic matter. The data collected, gathered with outputs from models of primary production, provide information aiming at having a holistic approach of the functioning of these habitats.

RESEARCHERS FROM THE TEAM BIOFEEL WORKING ON MUDFLATS :

Hélène Agogué
Pierrick Bocher
Christine Dupuy
Denis Fichet
Vincent Le Fouest
Benoit Lebreton

REFERENCE PUBLICATIONS :

Beninger P., 2018. Mudflat ecology. Springer, 429 p.
Day Jr. J.W., Hall C.A.S., Kemp M.W., Yáñez-Arancibia A., 1989. Estuarine ecology. John Wiley & Sons, New York, 558 p.
Kromkamp J.C., de Brouwer J.F.C., Blanchard G.F., Forster R., Créach V., 2006. Functioning of microphytobenthos in estuaries. Royal Academy of Arts and Sciences, Amsterdam, 262 p.
Mann K.H., 1982. Ecology of coastal waters : a systems approach. Blackwell Scientific Publications, 322 p.
McLusky D.S., 1989. The estuarine ecosystem. Chapman and Hall, New York, 256 p.

MAJOR PUBLICATIONS OF THE TEAM BIOFEEL :

Barnett A., Méléder V., Blommaert L., lepetit B., Gaudin P., Vyverman W., Sabbe K., Dupuy C., Lavaud J., 2015. Growth form defines physiological photoprotective capacity in intertidal benthic diatoms. The ISME Journal 9, 32-45.
Blanchard G.F., 2006. Analyse conceptuelle du système de production primaire microphytobenthique des vasières intertidales. Océanis 32, 215-235.
Bocher P., Piersma T., Dekinga A., Kraan C., Yates M.G., Guyot T., Folmer E.O., Radenac G., 2007. Site- and species-specific distribution patterns of molluscs at five intertidal soft-sediment areas in northwest Europe during a single winter. Marine Biology 151, 577-594.
Lavergne C., Agogué H., Leynaert A., Raimonet M., de Wit R., Pineau P., Bréret M., Lachaussée N., Dupuy C., 2017. Factors influencing prokaryotes in an intertidal mudflat and the resulting depth gradients. Estuarine, Coastal and Shelf Science, 189, 74-83.
Lebreton B., Rivaud A., Picot L., Prévost B., Barillé L., Sauzeau T., Beseres Pollack J., Lavaud J., 2019. From ecological relevance of the ecosystem services concept to its sociopolitical use. The case study of intertidal bare mudflats in the Marennes-Oléron Bay, France. Ocean and Coastal Management 172, 41-54.
Leguerrier D., Degré D., Niquil N., 2007. Network analysis and inter-ecosystem comparison of two intertidal mudflat food webs (Brouage Mudflat and Aiguillon Cove, SW France). Estuarine, Coastal and Shelf Science 74, 403-418.
Méléder V., Savelli R., Barnett A., Polsenaere P., Gernez P., Cugier P., Lerouxel A., Le Bris A., Dupuy C., Le Fouest V., Lavaud J., 2020. Mapping the intertidal microphytobenthos gross primary production, part I : Coupling multispectral remote sensing and physical modeling. Frontiers in Marine Science 7, 520.
Riera P., Richard P., 1996. Isotopic determination of food sources of Crassostrea gigas along a trophic gradient in the estuarine bay of Marennes-Oléron. Estuarine, Coastal and Shelf Science 42, 347-360.
Savelli R., Méléder V., Cugier P., Polsenaere P., Dupuy C., Lavaud J., Barnett A., Le Fouest V., 2020. Mapping the intertidal microphytobenthos gross Primary production, Part II : Merging remote sensing and physical-biological coupled modeling. Frontiers in Marine Science 7, 521
van der Heijden L.H., Niquil N., Haraldsson M., Asmus R.M., Pacella S.R., Graeve M., Rzeznik-Orignac J., Asmus H., Saint-Béat B., Lebreton B., 2020. Quantitative food web modeling unravels the importance of the microphytobenthos-meiofauna pathway for a high trophic transfer by meiofauna in soft-bottom intertidal food webs. Ecological Modelling 430, 109129.

Axis 1: Physiological and behavioural performances of animals in response to environmental variations

Tiffany Diague — 2018-07-25T13:28:32Z

Axis 1 works are about the study of physiological and behavioural responses at different organization levels (molecule, cell, organ, individual, population). In this context, some physiological functions of interest – immunity function, detoxification, breathing and filtration, cardiac function, energetic metabolism and locomotion – are favoured in order to better understand the mechanisms of their functioning, or the dysfunction toward environmental factors such as temperature, hypoxia, nutritional quality, acidification, or chemical contaminants. More precisely, we focus our works on the study of physiological processes having an ecological pertinence, via cellular physiology, biochemistry & functional genomics approaches, and individual approaches: behaviour, breathing, locomotion, growth.

Killifish (Nothobranchius furzeri)'s cardiac cell marked with DAPi + Phalloidine

These questions are constantly put back in an environmental context, taking account of the importance of abiotic (temperature), biotic (ontogenetic stage) and ecologic (spatio-temporal variations, change of ecosystem structure and trophic functioning) factors.

The aim of these works is multiple and tries to lead to:

The identification of causal relations between environmental forcing, biological mechanisms and individual performances
The understanding of the implications of physiological mechanisms in ecological processes
The definition of the thresholds of organisms' sensitivity toward environmental pressures
The definition of tolerance range in which occurs the physiologic functions necessary to maintain the organism's fitness

Escape of a startled golden grey mullet (images from a 500 frame/s high speed camera)

Some current projects:

Project Port de Plaisance La Rochelle: Improvement of environment quality (interannual follow-up) (2015-2018)
Project NITOXMOR: pseudo-nitzschia: toxicity or mortality? (2018)
Project PECTIMPACT (MAE/ Royal Norwegian Embassy): Multidisciplinary approach to PECTinidae Chlamys sp: IMPACT of a chemical contamination (2016-2019).
Project ECONAT (FEDER/CPER): port dynamics, urban environments and maritime environment DYPOMAR (2015-2020).

Participants of the axis:

Permanent staff: Anne Aubert ; Imbert Nathalie ; Lefrançois Christel ; Murillo Laurence ; Rosenfeld Eric ; Isabelle Goubet ; Thomas Helene ; Vagner Marie
CDD: N Ouillon (IE)
PhD students: Breitwieser Marine
Platforms: Ecophysiology, Aquarium, Swamp, Molecular Biology, Imaging Cytometry, High resolution analysis of biomolecules, Elementaries analysis

Axis 2: Potentiation of biological effects by vectorization, encapsulation and functionalization

Tiffany Diague — 2018-06-14T08:38:09Z

Our team's axis 2 is interested in developing and applying different methodologies aiming to improve biological effects of molecules produced in axis 1. Therefore, several strategies (functionalization, encapsulation, nanovectorization, actives combination) are studied.

Thematic 1: Potentiate anti-cancerous effects

Project 1: Modification of bioactive molecules by chemical/enzymatic functionalization

– Synthesis of small polynitrogen heterocyclic of different types such as diazaindole, oxindoles polynitrogen, thiazolopiridazoindazoles, targeting kinases (CDK, PI3Kα, haspine, or DYRK1A/CLK1) involved in the RNAm's splicing and carcinogenesis process. Valérie Thiery

Project 2: Conception of nano-objects for guided biotherapies of tumor microenvironment and diagnostic monitoring by clinic imaging

Key words: tumorous microenvironment, iron oxide nanoparticles, positive contrast MRI, vectorization, sulfated polysaccharides, heparanase inhibitors

– Conception and characterization of a new generation of iron oxide nanoparticles (IONP) extremely tiny, bio-conjugated to sulfated polysaccharides able to produce a positive contrast in magnetic resonance imaging (MRI). Hugo Groult

– Analysis in vivo of the bio-distribution of anti-tumorous nanovectors by MRI imaging among mice, and pharmacokinetics. Optimization towards bimodal diagnostic probes. (PET/MRI) Hugo Groult

Thematic 2: Potentiate cosmetic and nutraceutical effects

Project 1: Actives encapsulation / Formulation

– Elaboration of encapsulation systems with monitored release of actives coming from natural biopolymers of marine or terrestrial plants sources. Jérémy Carpentier (PhD student)

Thematic 3: Potentiate anti-microbial effects

Project 1: Combined action of molecules, synergy and restauration of sensitivity to antibiotics of pathogenic bacteria.

Project 2: Synthesis and modification by chemical means, or by genetic engineering of molecules of interest

Project 3: Characterization of the surface properties of supports on which anti-biofilm molecules can adhere.

Axis 1: discovery, identification and characterization of natural or bio-inspired bioactives molecules

Thierry Guyot — 2018-06-14T08:36:50Z

In this axis, skills in extraction, biotechnologies, and synthesis chemistry under microwave, are applied to different biomasses (micro/macro algae and marine bacteria), biological polymers or secondary metabolites. This way, different families of natural molecules or analog synthesis are obtained (glycomolecules, heterocyclic compounds, peptides, pigments, polyphenols) with the purpose to have anti-cancerous, cardio-metabolic, anti-microbial, and dermo-cosmetic applications.

The different skills of the team enable us to evaluate the molecules for their different biological activities: anti-proliferative, cytotoxic, regulators of tumorous progress's process (invasion, migration, angiogenesis), anti-microbial, anti-oxidant, anti-inflammatory… (on cellular lines or molecular targets).

These projects are also accompanied by the development of technological methods for the purification and the characterization (mass spectrometry-chromatography) of biological actives.

Thematic 1: Novel natural compounds with anti-cancer potential

Project 1: Natural pigments and alkaloid in oncology

Key words: marine micro-algae, pigments, pharmacology, cell signaling, kinase inhibitors

– Extraction, purification, characterization of biological activity of original molecules on a model of metastatic human melanoma and chemo-resistant, pharmacology of carotenoid pigments. Laurent Picot

– Molecular and cellular mechanisms occurring during cytotoxic effects of carotenoid pigments from micro algae. Audrey Fleury

– Identification of cytostatic molecules able to sensitize cancerous cells in order to potentiate treatments and decrease the resistance mechanisms toward anti-tumorous treatment. Raimundo Goncalves de Oliveira junior (PhD student)

– European project Interreg Enhance Microalgae: development of a database and an in-depth analysis of microalgae industrial promotion opportunities in the European Atlantic arc. Judith Rumin

– Conception and synthesis of new pharmacologic inhibitors of key kinases in carcinogenesis. Original synthesis of new indigoid's analogs. (diazaindoles, 3rd indolin-substitution-2-one). Valérie Thiery, Lisianne Domon, Jean-René Cherouvrier.

Project 2: Natural oligosaccharides in oncology

Key words: Cancer, tumorous microenvironment, neo-vascularization, sulfated oligosaccharides of natural origin, heparanase

– Polysaccharides' synthesis, structural mimes of sulfate heparan, inhibitors of heparanase, a glycoside hydrolase involved in pro-tumorous angiogenesis. Thierry Maugard, Hugo Groult

– Evaluation of in vitro biologic activity based on cellular models and in vivo based on mouse tumor models. This project is subject to a PhD, a post-doctoral and activities from researchers. Ingrid Arnaudin, Jean-Marie Piot, Isabelle Lanneluc, Grazielo Geneau, Rémi Cousin (PhD student)

Thematic 2: Novel natural compounds with nutraceutical and dermo-cosmetics potential

Key words: marine polysaccharides, marine peptides, metabolic syndrome, inflammation, insulin-resistance

– Valorization of macro-algae fractions obtained by the Seprosys method as active agents usable in the dermo-cosmetic and nutraceutical fields. This project is subject to a PhD and activities from researchers: Thierry Maugard,Nicolas Bridiau, Justine Bodin (PhD student)

– Sifting of marine peptides that can regulate some metabolic parameters associated with type 2 diabetes, dyslipidemia, arterial hypertension, and obesity. Stéphanie Bordenave

– Valorization of micro and macro-algae fragments for the development of actives targeting inflammation, and alteration mechanisms of the emotional behavior bound to metabolic disorders. Projet Neuronalg

– Valorization of exopolysaccharides from micro-algae for dermo-cosmetic and nutraceutical applications. This project is subject to a PhD and activities from researchers: Thierry Maugard, Nicolas Bridiau, Ingrid Arnaudin, Claire Toucheteau

Thematic 3: Novel natural compounds with anti-microbial potential

Key words: antibiofilm, metagenomics, non-ribosomal peptides, antibiotics

– Sifting of microbial stain of interest, producing inhibitors causing virulence (for instance antibiofilm) and/or growing. Isabelle Lanneluc, Sophie Sablé

– Identification of anti-microbial molecules, by the use of competitive phenomenons between microorganisms. Romain Chevrot, Sandrine Didelot, Cyrille Barthelemy

– Analysis of metagenomics banks and high-speed sequencing for the bioproduction of antibiotic non-ribosomal peptides. Conception and synthesis of innovative non-ribosomal peptides. Romain Chevrot, Cyrille Barthelemy, Caroline Salsano (PhD student)

– Sifting and identification of probiotic stems able to produce growth inhibitors. Sophie Sablé, Romain Chevrot, Sandrine Didelot, Sabrina Saltaji (PhD student).

Research axis

Thierry Guyot — 2018-06-14T08:33:43Z

The scientific project of the team occurs around two complementary axis in order to respond to the main challenges concerning the therapeutic arsenal renewal, the biology effects improvement, and the delivery of more specific bioactives molecules.

Axis 1: Discovery, identification and characterization of natural or bio-inspired bioactives molecules

Axis 2: Potentiation of biological effects by vectorization, encapsulation and functionalization

Scientific objectives

Thierry Guyot — 2018-06-14T08:32:47Z

Because of the apparition of resistance phenomenon's regarding current treatments of pathologies (cancer, microbial infections, metabolic diseases), and the too weak selectivity of the current molecules, there is a need for more research and for the development of new substances. In order to rise to these major challenges, the team's objective is the conception of pharmacological or nutritional actives of natural origin, mainly derived from or inspired by marine resources (micro- and macro-algae, bacteria).

In our team strategy, different molecular families are obtained (glycomolecules, peptides, polyphenols, pigments, heterocyclic compounds) by environmentally friendly methods, in accord with green chemistry. The biological aims and the selected targets (enzymes, receptors) are compounds with different functions such as antitumor, cardio metabolic regulators, anti-infective, and dermo-actives. These natural compounds, coming from marine organisms, plants, and agro-resources are placed in one of the 3 following banks, i.e. a chemical library, an extract bank, or a strain library. These three laboratory resource banks can be enhanced with new molecular structures by the sifting of isolated environmental resources (harvest's algae or culture‘s algae for instance) or complete ecosystems rich in microbial metabolisms that have a high biotechnological potential.

Scientific production from team "ESTRAN"

Thierry Guyot — 2014-03-05T10:22:51Z

publications 2023 :

33 results

2023

Article

Tritium Autrigonum (Monasterio de Rodilla, BURGOS): an urban approach to a Hispano-Roman agglomeration based on recent aerial surveys

Laurent Brassous , François Didierjean

Zephyrvs, 2023, 91, pp.103-140. ⟨10.14201/zephyrus202391103140⟩

LIttoral ENvironnement et Sociétés (LIENSs) - UMR 7266

benthic meiofauna

BENTHIC MEIOFAUNA :

BENTHIC MEIOFAUNA STUDIED IN THE TEAM BIOFEEL :

RESEARCH CARRIED OUT IN THE TEAM BIOFEEL ABOUT BENTHIC MEIOFAUNA :

RESEARCHERS FROM THE TEAM BIOFEEL WORKING ON BENTHIC MEIOFAUNA :

REFERENCE PUBLICATIONS :

MAJOR PUBLICATIONS OF THE TEAM BIOFEEL :

benthic microalgae

BENTHIC MICROALGAE :

BENTHIC MICROALGAE STUDIED IN THE TEAM BIOFEEL :

RESEARCH CARRIED OUT IN THE TEAM BIOFEEL ABOUT BENTHIC MICROALGAE :

RESEARCHERS FROM THE TEAM BIOFEEL WORKING ON BENTHIC MICROALGAE :

REFERENCE PUBLICATIONS :

MAJOR PUBLICATIONS OF THE TEAM BIOFEEL :

Mudflats

MUDFLATS : general description

MUDFLATS specifically studied by the researchers from the team BIOFEEL :

RESEARCH CARRIED OUR ON MUDFLATS :

RESEARCHERS FROM THE TEAM BIOFEEL WORKING ON MUDFLATS :

REFERENCE PUBLICATIONS :

MAJOR PUBLICATIONS OF THE TEAM BIOFEEL :

Axis 1: Physiological and behavioural performances of animals in response to environmental variations

Some current projects:

Participants of the axis:

Axis 2: Potentiation of biological effects by vectorization, encapsulation and functionalization

Thematic 1: Potentiate anti-cancerous effects

Project 1: Modification of bioactive molecules by chemical/enzymatic functionalization

Project 2: Conception of nano-objects for guided biotherapies of tumor microenvironment and diagnostic monitoring by clinic imaging

Thematic 2: Potentiate cosmetic and nutraceutical effects

Project 1: Actives encapsulation / Formulation

Thematic 3: Potentiate anti-microbial effects

Project 1: Combined action of molecules, synergy and restauration of sensitivity to antibiotics of pathogenic bacteria.

Project 2: Synthesis and modification by chemical means, or by genetic engineering of molecules of interest

Project 3: Characterization of the surface properties of supports on which anti-biofilm molecules can adhere.

Axis 1: discovery, identification and characterization of natural or bio-inspired bioactives molecules

Thematic 1: Novel natural compounds with anti-cancer potential

Project 1: Natural pigments and alkaloid in oncology

Project 2: Natural oligosaccharides in oncology

Thematic 2: Novel natural compounds with nutraceutical and dermo-cosmetics potential

Thematic 3: Novel natural compounds with anti-microbial potential

Research axis

Scientific objectives

Scientific production from team "ESTRAN"

2023

Article

Book

Book section

2022

Article

Book

Book section

2021

Article

Book

Book section

2020

Article

Book section

2019

Article

Book

Book section

2018

Article

Book

Book section

2017

Article

Book

Book section

Scientific production from team "BCBS"

2023

Article

2022

Article

Patent

2021

Article

Patent