LIttoral ENvironnement et Sociétés (LIENSs) - UMR 7266

Axis 2: Potentiation of biological effects by vectorization, encapsulation and functionalization

Tiffany Diague — 2018-06-14T08:38:09Z

Our team's axis 2 is interested in developing and applying different methodologies aiming to improve biological effects of molecules produced in axis 1. Therefore, several strategies (functionalization, encapsulation, nanovectorization, actives combination) are studied.

Thematic 1: Potentiate anti-cancerous effects

Project 1: Modification of bioactive molecules by chemical/enzymatic functionalization

– Synthesis of small polynitrogen heterocyclic of different types such as diazaindole, oxindoles polynitrogen, thiazolopiridazoindazoles, targeting kinases (CDK, PI3Kα, haspine, or DYRK1A/CLK1) involved in the RNAm's splicing and carcinogenesis process. Valérie Thiery

Project 2: Conception of nano-objects for guided biotherapies of tumor microenvironment and diagnostic monitoring by clinic imaging

Key words: tumorous microenvironment, iron oxide nanoparticles, positive contrast MRI, vectorization, sulfated polysaccharides, heparanase inhibitors

– Conception and characterization of a new generation of iron oxide nanoparticles (IONP) extremely tiny, bio-conjugated to sulfated polysaccharides able to produce a positive contrast in magnetic resonance imaging (MRI). Hugo Groult

– Analysis in vivo of the bio-distribution of anti-tumorous nanovectors by MRI imaging among mice, and pharmacokinetics. Optimization towards bimodal diagnostic probes. (PET/MRI) Hugo Groult

Thematic 2: Potentiate cosmetic and nutraceutical effects

Project 1: Actives encapsulation / Formulation

– Elaboration of encapsulation systems with monitored release of actives coming from natural biopolymers of marine or terrestrial plants sources. Jérémy Carpentier (PhD student)

Thematic 3: Potentiate anti-microbial effects

Project 1: Combined action of molecules, synergy and restauration of sensitivity to antibiotics of pathogenic bacteria.

Project 2: Synthesis and modification by chemical means, or by genetic engineering of molecules of interest

Project 3: Characterization of the surface properties of supports on which anti-biofilm molecules can adhere.

Axis 1: discovery, identification and characterization of natural or bio-inspired bioactives molecules

Thierry Guyot — 2018-06-14T08:36:50Z

In this axis, skills in extraction, biotechnologies, and synthesis chemistry under microwave, are applied to different biomasses (micro/macro algae and marine bacteria), biological polymers or secondary metabolites. This way, different families of natural molecules or analog synthesis are obtained (glycomolecules, heterocyclic compounds, peptides, pigments, polyphenols) with the purpose to have anti-cancerous, cardio-metabolic, anti-microbial, and dermo-cosmetic applications.

The different skills of the team enable us to evaluate the molecules for their different biological activities: anti-proliferative, cytotoxic, regulators of tumorous progress's process (invasion, migration, angiogenesis), anti-microbial, anti-oxidant, anti-inflammatory… (on cellular lines or molecular targets).

These projects are also accompanied by the development of technological methods for the purification and the characterization (mass spectrometry-chromatography) of biological actives.

Thematic 1: Novel natural compounds with anti-cancer potential

Project 1: Natural pigments and alkaloid in oncology

Key words: marine micro-algae, pigments, pharmacology, cell signaling, kinase inhibitors

– Extraction, purification, characterization of biological activity of original molecules on a model of metastatic human melanoma and chemo-resistant, pharmacology of carotenoid pigments. Laurent Picot

– Molecular and cellular mechanisms occurring during cytotoxic effects of carotenoid pigments from micro algae. Audrey Fleury

– Identification of cytostatic molecules able to sensitize cancerous cells in order to potentiate treatments and decrease the resistance mechanisms toward anti-tumorous treatment. Raimundo Goncalves de Oliveira junior (PhD student)

– European project Interreg Enhance Microalgae: development of a database and an in-depth analysis of microalgae industrial promotion opportunities in the European Atlantic arc. Judith Rumin

– Conception and synthesis of new pharmacologic inhibitors of key kinases in carcinogenesis. Original synthesis of new indigoid's analogs. (diazaindoles, 3rd indolin-substitution-2-one). Valérie Thiery, Lisianne Domon, Jean-René Cherouvrier.

Project 2: Natural oligosaccharides in oncology

Key words: Cancer, tumorous microenvironment, neo-vascularization, sulfated oligosaccharides of natural origin, heparanase

– Polysaccharides' synthesis, structural mimes of sulfate heparan, inhibitors of heparanase, a glycoside hydrolase involved in pro-tumorous angiogenesis. Thierry Maugard, Hugo Groult

– Evaluation of in vitro biologic activity based on cellular models and in vivo based on mouse tumor models. This project is subject to a PhD, a post-doctoral and activities from researchers. Ingrid Arnaudin, Jean-Marie Piot, Isabelle Lanneluc, Grazielo Geneau, Rémi Cousin (PhD student)

Thematic 2: Novel natural compounds with nutraceutical and dermo-cosmetics potential

Key words: marine polysaccharides, marine peptides, metabolic syndrome, inflammation, insulin-resistance

– Valorization of macro-algae fractions obtained by the Seprosys method as active agents usable in the dermo-cosmetic and nutraceutical fields. This project is subject to a PhD and activities from researchers: Thierry Maugard,Nicolas Bridiau, Justine Bodin (PhD student)

– Sifting of marine peptides that can regulate some metabolic parameters associated with type 2 diabetes, dyslipidemia, arterial hypertension, and obesity. Stéphanie Bordenave

– Valorization of micro and macro-algae fragments for the development of actives targeting inflammation, and alteration mechanisms of the emotional behavior bound to metabolic disorders. Projet Neuronalg

– Valorization of exopolysaccharides from micro-algae for dermo-cosmetic and nutraceutical applications. This project is subject to a PhD and activities from researchers: Thierry Maugard, Nicolas Bridiau, Ingrid Arnaudin, Claire Toucheteau

Thematic 3: Novel natural compounds with anti-microbial potential

Key words: antibiofilm, metagenomics, non-ribosomal peptides, antibiotics

– Sifting of microbial stain of interest, producing inhibitors causing virulence (for instance antibiofilm) and/or growing. Isabelle Lanneluc, Sophie Sablé

– Identification of anti-microbial molecules, by the use of competitive phenomenons between microorganisms. Romain Chevrot, Sandrine Didelot, Cyrille Barthelemy

– Analysis of metagenomics banks and high-speed sequencing for the bioproduction of antibiotic non-ribosomal peptides. Conception and synthesis of innovative non-ribosomal peptides. Romain Chevrot, Cyrille Barthelemy, Caroline Salsano (PhD student)

– Sifting and identification of probiotic stems able to produce growth inhibitors. Sophie Sablé, Romain Chevrot, Sandrine Didelot, Sabrina Saltaji (PhD student).

Research axis

Thierry Guyot — 2018-06-14T08:33:43Z

The scientific project of the team occurs around two complementary axis in order to respond to the main challenges concerning the therapeutic arsenal renewal, the biology effects improvement, and the delivery of more specific bioactives molecules.

Axis 1: Discovery, identification and characterization of natural or bio-inspired bioactives molecules

Axis 2: Potentiation of biological effects by vectorization, encapsulation and functionalization

Scientific objectives

Thierry Guyot — 2018-06-14T08:32:47Z

Because of the apparition of resistance phenomenon's regarding current treatments of pathologies (cancer, microbial infections, metabolic diseases), and the too weak selectivity of the current molecules, there is a need for more research and for the development of new substances. In order to rise to these major challenges, the team's objective is the conception of pharmacological or nutritional actives of natural origin, mainly derived from or inspired by marine resources (micro- and macro-algae, bacteria).

In our team strategy, different molecular families are obtained (glycomolecules, peptides, polyphenols, pigments, heterocyclic compounds) by environmentally friendly methods, in accord with green chemistry. The biological aims and the selected targets (enzymes, receptors) are compounds with different functions such as antitumor, cardio metabolic regulators, anti-infective, and dermo-actives. These natural compounds, coming from marine organisms, plants, and agro-resources are placed in one of the 3 following banks, i.e. a chemical library, an extract bank, or a strain library. These three laboratory resource banks can be enhanced with new molecular structures by the sifting of isolated environmental resources (harvest's algae or culture‘s algae for instance) or complete ecosystems rich in microbial metabolisms that have a high biotechnological potential.

Scientific production from team "BCBS"

Thierry Guyot — 2014-03-05T10:22:24Z

publications 2023 :

16 results

2023

Article

Role of some structural features in EPS from microalgae stimulating collagen production by human dermal fibroblasts

Claire Toucheteau , Valentine Deffains , Clément Gaignard , Christophe Rihouey , Céline Laroche , Guillaume Pierre , Olivier Lépine , Ian Probert , Didier Le Cerf , Philippe Michaud , Ingrid Arnaudin-Fruitier , Nicolas Bridiau , Thierry Maugard

Bioengineered, 2023, 14 (1), ⟨10.1080/21655979.2023.2254027⟩

LIttoral ENvironnement et Sociétés (LIENSs) - UMR 7266

Axis 2: Potentiation of biological effects by vectorization, encapsulation and functionalization

Thematic 1: Potentiate anti-cancerous effects

Project 1: Modification of bioactive molecules by chemical/enzymatic functionalization

Project 2: Conception of nano-objects for guided biotherapies of tumor microenvironment and diagnostic monitoring by clinic imaging

Thematic 2: Potentiate cosmetic and nutraceutical effects

Project 1: Actives encapsulation / Formulation

Thematic 3: Potentiate anti-microbial effects

Project 1: Combined action of molecules, synergy and restauration of sensitivity to antibiotics of pathogenic bacteria.

Project 2: Synthesis and modification by chemical means, or by genetic engineering of molecules of interest

Project 3: Characterization of the surface properties of supports on which anti-biofilm molecules can adhere.

Axis 1: discovery, identification and characterization of natural or bio-inspired bioactives molecules

Thematic 1: Novel natural compounds with anti-cancer potential

Project 1: Natural pigments and alkaloid in oncology

Project 2: Natural oligosaccharides in oncology

Thematic 2: Novel natural compounds with nutraceutical and dermo-cosmetics potential

Thematic 3: Novel natural compounds with anti-microbial potential

Research axis

Scientific objectives

Scientific production from team "BCBS"

2023

Article

2022

Article

Patent

2021

Article

Patent

2020

Article

Book section

Patent

2019

Article

Book section

2018

Article

Book section

Patent

2017

Article

Book section

Patent

Introduction